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What is the best way to naturally condition a 20 ltr keg other than with CO2?Is cane sugar OK,or something like maize syrup,better? And,can it be done at ambient room temperature?

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any sugar/fermentable syrup will do it, and yes it can be done at room temp. Just watch what fermentable you add as if it has a flavour of its own you may taste it in the finished brew.

I have carbonated a keg using dextrose, and that is also what i use for carbonating my bottles.

You will end up with a bunch of yeast sitting on the bottom of the keg just like you get a layer of yeast in the bottom of the bottle conditioned homebrew bottles. You can tilt the keg so the yeast settles away from the keg outpipe tho. Oh and I would not go moving the keg much unless you can sit it somewhere for a day or two for the yeast to settle again.

You can also naturally carbonate it -- transfer to the keg when you're about five points above your target final gravity. It will keep fermenting in the keg. As the CO2 has nowhere to go, it will carbonate the beer. Use aspunding valve to keep the carbonation to the right levels.

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